• Login
    View Item 
    •   FFI Publications Home
    • Publications
    • Articles
    • View Item
    •   FFI Publications Home
    • Publications
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Multichannel open tubular enzyme reactor online coupled with mass spectrometry for detecting ricin

    View/Open
    1521886.pdf (3.673Mb)
    Date
    2017
    Author
    Brandtzaeg, Ole Kristian
    Røen, Bent Tore
    Enger, Siri
    Lundanes, Elsa
    Wilson, Steven Ray Haakon
    Metadata
    Show full item record
    Abstract
    For counterterrorism purposes, a selective nano liquid chromatography–mass spectrometry (nanoLC-MS) platform was developed for detecting the highly lethal protein ricin from castor bean extract. Manual sample preparation steps were omitted by implementing a trypsin/Lys-C enzyme-immobilized multichannel reactor (MCR) consisting of 126 channels (8 μm inner diameter in all channels) that performed online digestion of proteins (5 min reaction time, instead of 4–16 h in previous in-solution methods). Reduction and alkylation steps were not required. The MCR allowed identification of ricin by signature peptides in all targeted mode injections performed, with a complete absence of carry-over in blank injections. The MCRs (interior volume ≈ 1 μL) have very low backpressure, allowing for trivial online coupling with commercial nanoLC-MS systems. The open tubular nature of the MCRs allowed for repeatable within/between-reactor preparation and performance.
    URI
    http://hdl.handle.net/20.500.12242/830
    https://ffi-publikasjoner.archive.knowledgearc.net/handle/20.500.12242/830
    DOI
    10.1021/acs.analchem.7b02590
    Description
    Brandtzaeg, Ole Kristian; Røen, Bent Tore; Enger, Siri; Lundanes, Elsa; Wilson, Steven Ray Haakon. Multichannel open tubular enzyme reactor online coupled with mass spectrometry for detecting ricin. Analytical Chemistry 2017 ;Volum 89.(17) s. 8667-8673
    Collections
    • Articles

    Browse

    All of FFI PublicationsCommunities & CollectionsBy Issue DateAuthorsTitlesThis CollectionBy Issue DateAuthorsTitles

    My Account

    Login

    CONTACT US

    • FFI Kjeller
      FFI, PO Box 25, 2027 Kjeller
    • Office Address: Instituttvn 20,
      Phone 63 80 70 00
    • biblioteket@ffi.no

    HELPFUL

    • About FFI
    • Career
    • Reports

    Sitemap

    • About cookies (cookies)
    • Newsletter
    • Sitemap

    FOLLOW US

     

     

    © Copyright Norwegian Defence Research Establishment
    Powered by KnowledgeArc