• Login
    View Item 
    •   FFI Publications Home
    • Publications
    • Articles
    • View Item
    •   FFI Publications Home
    • Publications
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Response of circulating immune cells to major gunshot injury, haemorrhage, and acute surgery

    View/Open
    991477.pdf (152.0Kb)
    Date
    2005
    Author
    Gundersen, Yngvar
    Vaagenes, Per
    Thrane, Ingjerd
    Bogen, Inger Lise
    Haug, Kristin Huse
    Reistad, Trine
    Opstad, Per Kristian
    Metadata
    Show full item record
    Abstract
    Purpose: The purpose of this study was to use an established porcine model to investigate the effects on immune function of severe gunshot injury. Methods: Twelve pigs sustained two standardised rounds, one through right femur and one through left upper abdomen. First aid treatment and acute surgery was started immediately. Blood samples were drawn before shooting and after 75 min. Circulating neutrophils were isolated and reactive oxygen species (ROS) measured. Serum levels of tumour necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and IL-10 were determined at 0, 75 min, as well as 2 h after incubation with 1 μg/ml endotoxin in an ex vivo whole blood model. Results: TNF-α, IL-1β, and IL-6 significantly increased at 75 min. ROS in circulating granulocytes tended to increase (NS). Incubation with endotoxin led to a more than 100-fold increase of TNF-α pre-trauma, compared to a three-fold increase post-trauma (p < 0.0001 between groups). A similar pattern was obtained for IL-1β, and IL-6. IL-10 was below detection in all samples. The granulocytes maintained their ability to react to the protein kinase C activator phorbol myristate acetate (PMA) after trauma. Conclusion: Severe gunshot injury and peritraumatic stress rapidly activate circulating immune cells, but reduce their capacity to react to a subsequent challenge to endotoxin.
    URI
    http://hdl.handle.net/20.500.12242/665
    https://ffi-publikasjoner.archive.knowledgearc.net/handle/20.500.12242/665
    DOI
    10.1016/j.injury.2004.09.021
    Description
    Gundersen, Yngvar; Vaagenes, Per; Thrane, Ingjerd; Bogen, Inger Lise; Haug, Kristin Huse; Reistad, Trine; Opstad, Per Kristian. Response of circulating immune cells to major gunshot injury, haemorrhage, and acute surgery. Injury 2005 ;Volum 36.(8) s. 949-955
    Collections
    • Articles

    Browse

    All of FFI PublicationsCommunities & CollectionsBy Issue DateAuthorsTitlesThis CollectionBy Issue DateAuthorsTitles

    My Account

    Login

    CONTACT US

    • FFI Kjeller
      FFI, PO Box 25, 2027 Kjeller
    • Office Address: Instituttvn 20,
      Phone 63 80 70 00
    • biblioteket@ffi.no

    HELPFUL

    • About FFI
    • Career
    • Reports

    Sitemap

    • About cookies (cookies)
    • Newsletter
    • Sitemap

    FOLLOW US

     

     

    © Copyright Norwegian Defence Research Establishment
    Powered by KnowledgeArc