• Login
    View Item 
    •   FFI Publications Home
    • Publications
    • Articles
    • View Item
    •   FFI Publications Home
    • Publications
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Identification and quantification of lichenysin – a possible source of food poisoning

    View/Open
    1278534.pdf (806.4Kb)
    Date
    2015
    Author
    Rønning, Helene Thorsen
    Madslien, Elisabeth Henie
    Asp, Tone Normann
    Granum, Per Einar
    Metadata
    Show full item record
    Abstract
    Lichenysin produced by 53 different Bacillus licheniformis strains has been structurally examined with a qualitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) method using quadrupole-time-of-flight mass spectrometry. The same lichenysin isoforms are produced from all strains, indicating that the growth conditions have a stronger influence on the lipopeptide production than the genotype. A rapid method for the quantification of lichenysin from bacterial cell cultures with LC-MS/MS after a simple methanol extraction has been refined. For the first time commercially available lichenysin has been used as calibrant, making quantification more accurate. The trueness for C15-lichenysin has been improved to 94% using matrix-matched calibration with lichenysin compared with 30% using solvent calibration with surfactin. The quantitative method was fully validated based on Commission Decision 2002/657/EC. The LOD of the method was below 1 µg g–1 and the repeatability ranged from 10% to 16%.
    URI
    https://ffi-publikasjoner.archive.knowledgearc.net/handle/20.500.12242/495
    DOI
    10.1080/19440049.2015.1096967
    Description
    Rønning, Helene Thorsen; Madslien, Elisabeth Henie; Asp, Tone Normann; Granum, Per Einar. Identification and quantification of lichenysin – a possible source of food poisoning. Food Additives & Contaminants 2015 ;Volum 32.(12) s. 2120-2130
    Collections
    • Articles

    Browse

    All of FFI PublicationsCommunities & CollectionsBy Issue DateAuthorsTitlesThis CollectionBy Issue DateAuthorsTitles

    My Account

    Login

    CONTACT US

    • FFI Kjeller
      FFI, PO Box 25, 2027 Kjeller
    • Office Address: Instituttvn 20,
      Phone 63 80 70 00
    • biblioteket@ffi.no

    HELPFUL

    • About FFI
    • Career
    • Reports

    Sitemap

    • About cookies (cookies)
    • Newsletter
    • Sitemap

    FOLLOW US

     

     

    © Copyright Norwegian Defence Research Establishment
    Powered by KnowledgeArc