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    Large scale MALDI-TOF MS based taxa identification to identify novel pigment producers in a marine bacterial culture collection

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    1009804.pdf (601.8Kb)
    Date
    2013
    Author
    Stafsnes, Marit Hallvardsd
    Dybwad, Marius
    Brunsvik, Anders
    Bruheim, Per
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    Abstract
    A challenge in the rational exploitation of microbial culture collections is to avoid superfluous testing of replicas. MALDI-TOF MS has been shown to be an efficient dereplication tool as it can be used to discriminate between bacterial isolates at the species level. A bacterial culture collection of more than 10,000 heterotrophic marine bacterial isolates from sea-water surface layers of the Norwegian Trondheimsfjord and neighbouring coastal areas has been established. A sub-collection of pigmented isolates was earlier screened for novel carotenoids with UVA-Blue light absorbing properties. This was a comprehensive analytical task and it was observed that a significant number of extracts with identical pigment profile were recovered. Hence, this study was undertaken to explore the use of MALDI-TOF MS as a dereplication tool to quickly characterize the bacterial collection. Furthermore, LC-DAD-MS analysis of pigment profiles was performed to check if pigment profile diversity was maintained among isolates kept after the potential MALDI-TOF MS selection step. Four hundred isolates comprising both pigmented and non-pigmented isolates were used for this study. The resulting MALDI-TOF MS dendrogram clearly identified a diversity of different taxa and these were supported by the pigment profile clustering, thus linking the pigment production as species-specific properties. Although one exception was found, it can be concluded that MALDI-TOF MS dereplication is a promising pre-screening tool for more efficient screening of microbial culture collection containing pigments with potential novel properties.
    URI
    https://ffi-publikasjoner.archive.knowledgearc.net/handle/20.500.12242/469
    DOI
    10.1007/s10482-012-9844-6
    Description
    Stafsnes, Marit Hallvardsd; Dybwad, Marius; Brunsvik, Anders; Bruheim, Per. Large scale MALDI-TOF MS based taxa identification to identify novel pigment producers in a marine bacterial culture collection. Antonie van Leeuwenhoek. International Journal of General and Molecular Microbiology 2013 ;Volum 103.(3) s. 603-615
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