• Login
    View Item 
    •   FFI Publications Home
    • Publications
    • Articles
    • View Item
    •   FFI Publications Home
    • Publications
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Role of the gerA operon in L-alanine germination of Bacillus licheniformis spores

    View/Open
    931291.pdf (512.1Kb)
    Date
    2012
    Author
    Løvdal, Irene Stranden
    From, Cecilie
    Madslien, Elisabeth
    Romundset, Kristin Cecilia Saue
    Klufterud, Elin
    Rosnes, Jan Thomas
    Granum, Per Einar
    Metadata
    Show full item record
    Abstract
    Background The genome of Bacillus licheniformis DSM 13 harbours three neighbouring open reading frames showing protein sequence similarities to the proteins encoded from the Bacillus subtilis subsp. subtilis 168 gerA operon, GerAA, GerAB and GerAC. In B. subtilis, these proteins are assumed to form a germinant receptor involved in spore germination induced by the amino acid L-alanine. Results In this study we show that disruption of the gerAA gene in B. licheniformis MW3 hamper L-alanine and casein hydrolysate-triggered spore germination, measured by absorbance at 600 nm and confirmed by phase contrast microscopy. This ability was restored by complementation with a plasmid-borne copy of the gerA locus. Addition of D-alanine in the casein hydrolysate germination assay abolished germination of both B. licheniformis MW3 and the complementation mutant. Germination of both B. licheniformis MW3 and the gerA disruption mutant was induced by the non-nutrient germinant Ca2+-Dipicolinic acid. Conclusions These results demonstrate that the B. licheniformis MW3 gerA locus is involved in germination induced by L-alanine and potentially other components present in casein hydrolysate.
    URI
    https://ffi-publikasjoner.archive.knowledgearc.net/handle/20.500.12242/17
    DOI
    10.1186/1471-2180-12-34
    Description
    Løvdal, Irene Stranden; From, Cecilie; Madslien, Elisabeth; Romundset, Kristin Cecilia Saue; Klufterud, Elin; Rosnes, Jan Thomas; Granum, Per Einar. Role of the gerA operon in L-alanine germination of Bacillus licheniformis spores. BMC Microbiology 2012 ;Volum 12.
    Collections
    • Articles

    Browse

    All of FFI PublicationsCommunities & CollectionsBy Issue DateAuthorsTitlesThis CollectionBy Issue DateAuthorsTitles

    My Account

    Login

    CONTACT US

    • FFI Kjeller
      FFI, PO Box 25, 2027 Kjeller
    • Office Address: Instituttvn 20,
      Phone 63 80 70 00
    • biblioteket@ffi.no

    HELPFUL

    • About FFI
    • Career
    • Reports

    Sitemap

    • About cookies (cookies)
    • Newsletter
    • Sitemap

    FOLLOW US

     

     

    © Copyright Norwegian Defence Research Establishment
    Powered by KnowledgeArc