NavLab OneClick is a MatLab script for automatic NavLab pre-processing, estimation, and export of finished smoothed data. This means that the user will be relieved of data structure creation, wild point filtering, estimator initialisation, quality control, and export of the smoothed results. NavLab OneClick is a shell surrounding NavLab, performing many of the labouring tasks that otherwise requires user intervention. This report holds the user guide for OneClick, including description of the necessary set up required before running NavLab OneClick the first time. All user defined parameters used by OneClick is thoroughly described. One chapter is devoted to a discussion on memory constraints, and some suggestions on how to improve the computer’s performance. Finally the report gives a more detailed description of the code, what it does, and why it does it.