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dc.contributor.authorEllingsen, Anette Bauer
dc.contributor.authorOlsen, Jaran Strand
dc.contributor.authorGranum, Per Einar
dc.contributor.authorRørvik, Liv Marit
dc.contributor.authorGonzalez-Escalona, Narjol
dc.date.accessioned2017-09-28T09:09:33Z
dc.date.accessioned2017-09-29T08:27:28Z
dc.date.available2017-09-28T09:09:33Z
dc.date.available2017-09-29T08:27:28Z
dc.date.issued2013
dc.identifier.citationEllingsen, Olsen JS, Granum PE, Rørvik LM, Gonzalez-Escalona. Genetic characterization of trh positive Vibrio spp. isolated from Norway. Frontiers in Cellular and Infection Microbiology. 2013;3en_GB
dc.identifier.urihttp://hdl.handle.net/20.500.12242/645
dc.identifier.urihttps://ffi-publikasjoner.archive.knowledgearc.net/handle/20.500.12242/645
dc.descriptionEllingsen, Anette Bauer; Olsen, Jaran Strand; Granum, Per Einar; Rørvik, Liv Marit; Gonzalez-Escalona, Narjol. Genetic characterization of trh positive Vibrio spp. isolated from Norway. Frontiers in Cellular and Infection Microbiology 2013 ;Volum 3.en_GB
dc.description.abstractThe thermostable direct hemolysin (TDH) and/or TDH-related hemolysin (TRH) genes are carried by most virulent Vibrio parahaemolyticus serovars. In Norway, trh+ V. parahaemolyticus constitute 4.4 and 4.5% of the total number of V. parahaemolyticus isolated from blue mussel (Mytilus edulis) and water, respectively. The trh gene is located in a region close to the gene cluster for urease production (ure). This region was characterized in V. parahaemolyticus strain TH3996 and it was found that a nickel transport operon (nik) was located between the first gene (ureR) and the rest of the ure cluster genes. The organization of the trh-ureR-nik-ure gene cluster in the Norwegian trh+ isolates was unknown. In this study, we explore the gene organization within the trh-ureR-nik-ure cluster for these isolates. PCR analyses revealed that the genes within the trh-ureR-nik-ure gene cluster of Norwegian trh+ isolates were organized in a similar fashion as reported previously for TH33996. Additionally, the phylogenetic relationship among these trh+ isolates was investigated using Multilocus Sequence Typing (MLST). Analysis by MLST or ureR-trh sequences generated two different phylogenetic trees for the same strains analyzed, suggesting that ureR-trh genes have been acquired at different times in Norwegian V. parahaemolyticus isolates. MLST results revealed that some pathogenic and non-pathogenic V. parahaemolyticus isolates in Norway appear to be highly genetically related.en_GB
dc.language.isoenen_GB
dc.titleGenetic characterization of trh positive Vibrio spp. isolated from Norwayen_GB
dc.typeArticleen_GB
dc.date.updated2017-09-28T09:09:33Z
dc.identifier.cristinID1102805
dc.identifier.cristinID1102805
dc.identifier.doi10.3389/fcimb.2013.00107
dc.source.issn2235-2988
dc.type.documentJournal article
dc.relation.journalFrontiers in Cellular and Infection Microbiology


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